Spatial Organization of the Cytoskeleton enhances Cargo Delivery to Specific Target Areas on the Plasma Membrane of Spherical Cells

Intracellular transport is vital for the proper functioning and survival of a cell. Cargo (proteins, vesicles, organelles, etc.) is transferred from its place of creation to its target locations via molecular motor assisted transport along cytoskeletal filaments. The transport efficiency is strongly affected by the spatial organization of the cytoskeleton, which constitutes an inhomogeneous, complex network. In cells with a centrosome microtubules grow radially from the central microtubule organizing center towards the cell periphery whereas actin filaments form a dense meshwork, the actin cortex, underneath the cell membrane with a broad range of orientations. The emerging ballistic motion along filaments is frequently interrupted due to constricting intersection nodes or cycles of detachment and reattachment processes in the crowded cytoplasm. In order to investigate the efficiency of search strategies established by the cell's specific spatial organization of the cytoskeleton we formulate a random velocity model with intermittent arrest states. With extensive computer simulations we analyze the dependence of the mean first passage times for narrow escape problems on the structural characteristics of the cytoskeleton, the motor properties and the fraction of time spent in each state. We find that an inhomogeneous architecture with a small width of the actin cortex constitutes an efficient intracellular search strategy.Comment: 14 pages, 9 figure

Crossovers in the Two Dimensional Ising Spin Glass with ferromagnetic next-nearest-neighbor interactions

By means of extensive computer simulations we analyze in detail the two dimensional $\pm J$ Ising spin glass with ferromagnetic next-nearest-neighbor interactions. We found a crossover from ferromagnetic to ``spin glass'' like order both from numerical simulations and analytical arguments. We also present evidences of a second crossover from the ``spin glass'' behavior to a paramagnetic phase for the largest volume studied.Comment: 19 pages with 9 postscript figures also available at http://chimera.roma1.infn.it/index_papers_complex.html. Some changes in captions of figures 1 and

Particle acceleration close to the supermassive black hole horizon: the case of M87

The radio galaxy M87 has recently been found to be a rapidly variable TeV emitting source. We analyze the implications of the observed TeV characteristics and show that it proves challenging to account for them within conventional acceleration and emission models. We discuss a new pulsar-type scenario for the origin of variable, very high energy (VHE) emission close to the central supermassive black hole and show that magneto-centrifugally accelerated electrons could efficiently Compton upscatter sub-mm ADAF disk photons to the TeV regime, leading to VHE characteristics close to the observed ones. This suggests, conversely, that VHE observations of highly under-luminous AGNs could provide an important diagnostic tool for probing the conditions prevalent in the inner accretion disk of these sources.Comment: 5 pages, one figure (typos corrected); based on presentation at "High Energy Phenomena in Relativistic Outflows", Dublin, Sept. 2007; accepted for publication in International Journal of Modern Physics

Nonequilibrium Dynamics and Aging in the Three--Dimensional Ising Spin Glass Model

The low temperature dynamics of the three dimensional Ising spin glass in zero field with a discrete bond distribution is investigated via MC simulations. The thermoremanent magnetization is found to decay algebraically and the temperature dependent exponents agree very well with the experimentally determined values. The nonequilibrium autocorrelation function $C(t,t_w)$ shows a crossover at the waiting (or {\em aging}) time $t_w$ from algebraic {\em quasi-equilibrium} decay for times $t$$\ll$$t_w$ to another, faster algebraic decay for $t$$\gg$$t_w$ with an exponent similar to one for the remanent magnetization.Comment: Revtex, 11 pages + 4 figures (included as Latex-files

Maternal Interactive Behaviors and Developmental Outcomes in Preschoolers Born Very Low Birth Weight

Children born very low birth weight (VLBW) are at greater risk for cognitive and executive deficits than children born normal birth weight. One factor in development is parental wellbeing. Parents who report more depressive symptoms and lower perception of resources have poorer quality interactive behaviors. This study investigated the associations between maternal interactive behaviors during play and developmental outcomes in preschoolers born VLBW. A secondary goal was to understand the associations among maternal depressive symptoms, perception of resources, and interactive behaviors. A tertiary goal was to investigate racial and ethnic differences in maternal behaviors. Overall, one maternal behavior was negatively associated with a measure of executive functioning. Maternal depressive symptoms were negatively correlated with one maternal behavior and maternal perception of resources was negatively correlated with another maternal behavior. Additional analyses found a trend in racial and ethnic differences in attention directing behaviors between Caucasian and Native American mothers

Evaluation of a microbial muramidase supplementation on growth performance, apparent ileal digestibility, and intestinal histology of broiler chickens

The current study evaluated the effects of different inclusion levels of microbial muramidase (Muramidase 007, DSM Nutritional Products) on gastrointestinal functionality by determination of apparent ileal digestibility (AID) of nutrients, investigation of intestinal histomorphology, and quantification of resulting growth performance. Four maize-wheat-soybean experimental diets were produced without (C) and with different dosages of muramidase: low (L, 25,000 LSU(F)/kg), medium (M, 35,000 LSU(F)/kg), and high (H, 45,000 LSU(F)/kg); diets were fed to broilers for 35 d. At the end of the experiment, AID of ether extract (EE), crude protein (CP), Ca, and P were determined and samples of the mid-jejunum and -ileum were collected for histomorphological observations. Data were subjected to ANOVA analysis using the GLM procedure. Orthogonal polynomial contrasts were used to assess linear and quadratic effects of different levels of the muramidase. At the end of the trial, Muramidase 007 supplementation linearly increased body weight gain and decreased feed conversion ratio (FCR) (P ≤ 0.05). Adding the muramidase to broiler diets also linearly increased the European poultry efficiency factor (P ≤ 0.05). Inclusion of the muramidase in broiler diets linearly increased AID of CP, EE, and P (P ≤ 0.05), and the H group had a higher AID of EE and CP compared to C group (P ≤ 0.05). Microbial muramidase supplementation linearly increased ileal villus length to crypt depth ratio and decreased the number of ileal CD45 cells (P ≤ 0.05). Broilers fed M and H diets had fewer number of CD45 cells in the ileum compared to those in C group (P ≤ 0.05). In conclusion, the results of the present study demonstrated that inclusion of the microbial muramidase in broiler diets could increase AID of key nutrients and improve growth performance in broilers. Adding microbial muramidase to broiler diets can therefore be considered as an interesting prospect to improve gastrointestinal functionality. Biological mechanisms causing these improvements need to be studied further